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Taq DNA polymerase, from Thermus aquaticus, is the standard PCR enzyme. It is robust, inexpensive and thermostable. It also has one weakness: it is not completely inactive at low temperatures.
While you assemble reactions at room temperature or on the bench, Taq retains low but real activity. Primers can anneal loosely to each other or to off-target sites, and the enzyme extends them. These mis priming products and primer-dimers are then amplified efficiently in every later cycle. They compete with your target for primers, dNTPs and enzyme, which lowers yield and sensitivity.
Regular Taq performs well when:
| Feature | Regular Taq | HotStart Taq |
|---|---|---|
| Activity at room temperature | Low but present | Blocked until heat activation |
| Primer-dimers / non-specific products | More likely | Significantly reduced |
| Sensitivity with low-copy template | Moderate | Higher |
| Multiplex PCR | Challenging | Better suited |
| Reaction setup flexibility | Ice recommended | Room-temperature setup possible |
| Initial activation step | Not needed | Required (check datasheet) |
| Cost | Lower | Higher |
| Product | Product Code | Pack | Price (₹) | Product Link |
|---|---|---|---|---|
| Taq DNA Polymerase (3 U/µL), Buffer A | MME5L | 250 U | 3,470 | View product › |
| Taq DNA Polymerase (3 U/µL), Buffer A | MME5J | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (3 U/µL), Buffer B + 25 mM MgCl₂ | MME25J | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (1 U/µL), Buffer A | MME24L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (1 U/µL), Buffer B + 25 mM MgCl₂ | MME28L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (1 U/µL), Buffer E (Triton X-100) | MME31L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (1 U/µL), Buffer F (Triton X-100) + 25 mM MgCl₂ | MME34L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer A | MME23L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer A | MME23B | 5000 U | 26,890 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer B + 25 mM MgCl₂ | MME27L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer B + 25 mM MgCl₂ | MME27B | 5000 U | 26,890 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer E (Triton X-100) | MME30L | 1000 U | 5,730 | View product › |
| Taq DNA Polymerase (5 U/µL), Buffer F (Triton X-100) + 25 mM MgCl₂ | MME33L | 1000 U | 5,730 | View product › |
| GeNei™ PCR Master Mix (2X) | MME22S | 100 rxns | 3,790 | View product › |
| GeNei™ Red Dye PCR Master Mix (2X) | MME21S | 100 rxns | 3,790 | View product › |
| GeNei™ Red Dye PCR Master Mix (2X) | MME21 | 5 x 100 rxns | 13,280 | View product › |
| GeNei™ Red Dye PCR Master Mix (2X) | MME21L | 2000 rxns | 40,780 | View product › |
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